E mission inc MICROSPHERES
Both familiar and superior
MagenTx 50 microspheres are built on a core particle of a proprietary polymer composition. These core particles are evenly coated with a uniform layer of ultrafine, superparamagnetic nanocrystalline magnetite incorporated into a carboxylate functionalized polymer. This unique configuration results in a highly magnetically responsive microsphere with a density slightly greater than typical biological buffers, which allows isolation by centrifugation or magnetization while still exhibiting dramatically slower settling under ambient conditions.
With these innovative features, the MagenTx 50 microspheres are easier to work with than any other magnetic microsphere. Coupling to the COOH groups on the surface is achieved by any number of well-known protocols, and the slow settling rate will ensure that the microspheres stay well dispersed in solution for the duration of most procedures. The result is a more even, uniform coupling of biological molecules to the entire microsphere population.
LOW DENSITY MAGNETIC MICROSPHERES PROVIDE A NUMBER OF BENEFITS:
Slow settling during coupling leads to improved uniformity of reagents on the microsphere surface
With approximately 5% magnetite and up to 90% surface coverage, the microspheres respond rapidly to a magnetic field
By staying in solution longer the MagenTx 50 microspheres display improved and more uniform reaction with targets in solution
Lower density microspheres allow for easier dispersion of pelleted microspheres
Slow settling also improves the uniformity of plate reads – no decreased microsphere counts when reading from wells 1 to 384.
LEGACY MAGNETIC MICROSPHERES
Our microspheres were designed to have a markedly smoother surface, as compared to other commonly available magnetic microspheres shown on the right. Rough outer surfaces are thought to contribute to high non-specific adsorption and other undesirable performance characteristics. By smoothing out the “hills and valleys” on the surface MagenTx 50 microspheres display a more uniform reactive surface.